Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides |
RNP only, Female |
Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells. |
epithelium of main bronchus |
respiratory epithelial cell |
Editing Efficiency |
15000001241 |
|
SpCas9 |
|
|
SpyCas9 g-loxP2_C9 |
|
|
Mouse |
B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J |
|
intranasal |
airway epithelium |
2.5 micromolar SpCas9, 2 micromolar RNA |
twice, one day apart |
|
55 microliters |
1 week |
ribonucleoprotein (RNP) |
|
|
|
Measured Values |
Samples Size: | 2 |
|
Mean |
0.0  |
1 |
0  |
2 |
0  |
| |
|