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Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
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Experiment Record Detail
Experiment
Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Experiment Condition
Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA
Assay Description:
Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.
Tissue Measured:
scala tympani
Measurement Type:
Editing Efficiency
Record ID
15000001309
Editor
Delivery System
306-O12B blank
Delivery System Subtype
Nanoparticle
Guide
Vector
Model Species
Mouse
Model Name
B6.Cg-Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze/J
Application Method
canalostomy injection
Application Site
inner ear
Dosage
Injection Frequency
once
Injection Rate
Injection Volume
1 microliter
Days post injection
6 days
Editor Format
Lipid Nanoparticle
Antidote Id
Antidote Description
Measured Values
Samples Size:
2
Replicate
Result (Editing Efficiency)
Measurement Units:
Signal
Mean
2 of 2 absent 
1
absent 
2
absent