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ExperimentRepeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Experiment ConditionControl (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA
Assay Description: Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.
Tissue Measured:scala tympani
Measurement Type:Editing Efficiency
Record ID15000001309

Editor
Delivery System306-O12B blank
Delivery System SubtypeNanoparticle
Guide
Vector

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze/J

Application Methodcanalostomy injection
Application Siteinner ear
Dosage
Injection Frequencyonce
Injection Rate
Injection Volume1 microliter
Days post injection6 days
Editor FormatLipid Nanoparticle
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: Signal
Mean 2 of 2 absent 
1 absent 
2 absent