Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

Validation Experiment: Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

PI: Stephen A Murray, PhD

Description: Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice

Editing Assay: Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.

Parent Project: The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi)

Download: Submitted files

Original Experiment/s that are being validated:

Tissues

Base of cochlear canal(TARGET)

Scala tympani(TARGET)

Cell Types

Unspecified

Editors

CleanCap® Cas9 mRNA (5moU)

Guide Target Locus

Ai9/Ai14 reporter transgene

Guides

sg298

Delivery Systems

306-O12B

306-O12B blank

Models

Ai14 mouse (congenic)

Sex

Male

Repeat_experiment_of_independent_validation_of_Chen_delivery_platform_using_LNPs_to_deliver_CRISPR/Cas9_to_mouse_inner_ear_
											Editing Efficiency
Record Id	Condition	Tissue	Cell Type	Sex	Age	Editor	Model	Delivery	Target Locus	Guide	Injection Frequency	Editing Efficiency Signal	Image
15000001306	Arm 1 (Repeat 4/20/21): 306-O12B LNP containing Cas9 mRNA and sgRNA	base of cochlear canal (TARGET)			8 weeks	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-O12B	Ai9/Ai14 reporter transgene	sg298	once	Present; absent; absent; absent; absent; absent;
15000001307	Arm 1 (Repeat 4/20/21): 306-O12B LNP containing Cas9 mRNA and sgRNA	scala tympani (TARGET)			8 weeks	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-O12B	Ai9/Ai14 reporter transgene	sg298	once	Present; absent; absent; absent; absent; absent;
15000001308	Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA	base of cochlear canal (TARGET)		Male	8 weeks		Ai14 mouse (congenic)	306-O12B blank			once	absent; absent;
15000001309	Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA	scala tympani (TARGET)		Male	8 weeks		Ai14 mouse (congenic)	306-O12B blank			once	absent; absent;