Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

PI: Stephen A Murray, PhD

Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice

Editing Assay:

- Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.

Organ System Overview

Arm 1 (Repeat 4/20/21): 306-O12B LNP containing Cas9 mRNA and sgRNA

Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA

Legend:

Analyze Data Sets Available for this Experiment
Sensory
	Base of cochlear canal (unspecified)
	Scala tympani (unspecified)

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