Experiment Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Experiment Condition Arm 1b: BI28 Neuron Specific Promoter, Positive Delivery Control Animals, Males Assay Description: Identification of of GFP-positive nuclei (green) Tissue Measured: brain Cell Type : neuron Measurement Type: Delivery Efficiency Record ID 15000001452 Editor SaCas9 Delivery System Delivery System Subtype Guide Vector BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method Application Site brain Dosage 0.25E+11 viral genomes/gram for each virus Injection Frequency once Injection Rate Injection Volume 5 microliters/gram Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 4 of 4 present  1 present  2 present  3 present  4 present