Experiment Validating Gene Editing Reporter 12 (GER12) Mouse Model in Heterozygous Blastocysts Experiment Condition GER12-g1-FWD Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express Katushka2S (RFP) out of the total blasts imaged. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000003589 Editor Alt-R™ S.p. Cas9 Nuclease V3 Delivery System Delivery System Subtype Guide GER12-g1-FWD Vector Model Species Mouse Model Name GER12 Heterozygous Blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: % of blasts with RFP activation Mean 52.09  1 42.86  2 50  3 63.41  Measurement Units: % of blasts with edits Mean 89.03  1 100  2 78.05  Measurement Units: Signal Mean # blasts analyzed  1 58 blasts for RFP  Mean # blasts analyzed  1 51 blasts for edits