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Editors
 ABE7.10
Guide Target Locus
 Venus* Q81X
Guides
 GER10-ABE-REV
Models
 GER10 Heterozygous Blastocyst
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Results

Validating_Gene_Editing_Reporter_10_(GER10)__Mouse_Model_in_Heterozygous_Blastocysts
Editing Efficiency
Record Id Condition Editor Model Target Locus Guide Editing Efficiency Signal Editing Efficiency % of blasts with Venus activation Editing Efficiency % of blasts with edits Image
15000003447 ABE ABE7.10 GER10 Heterozygous Blastocyst Venus* Q81X GER10-ABE-REV

Validation of Gene Editing Reporter 10 (GER10) Mouse Model in Heterozygous Blastocysts

Zygotes were electroporated with Cas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Venus fluorescence and scored for fluorescence signal.