Experiment Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway Experiment Condition S10 and ABE8e-Cas9 RNP Assay Description: Editing efficiency was assessed using Next Generation Sequencing of airway epithelial cells obtained by airway brushing in two airways of the airway tree Tissue Measured: lobar bronchus of right lung middle lobe Cell Type : bronchial epithelial cell Measurement Type: Editing Efficiency Record ID 15000002998 Editor ABE8e-Cas9 Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide gRNA #8 Vector Model Species Rhesus macaque Model Name Macaca mulatta (Rhesus Macaque) Application Method intra-tracheal instillation Application Site airway epithelium Dosage 2 uM gRNA, 2.5 uM Cas9, 40 uM S10 amphiphilic peptide Injection Frequency once Injection Rate n/a Injection Volume 1 ml Days post injection 7 days Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % of A•T basepairs converted to G•C Mean 0.03  1 0.025