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ExperimentAmphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Experiment ConditionS10 and DRI-NLS-Cy5 (10 uM)
Assay Description: Editing efficiency was assessed using Next Generation Sequencing of airway epithelial cells obtained by airway brushing in two airways of the airway tree
Tissue Measured:trachea
Cell Type :tracheal epithelial cell
Measurement Type:Editing Efficiency
Record ID15000003030

Editor
Delivery SystemS10
Delivery System SubtypeAmphiphilic peptide
Guide
Vector

Model SpeciesRhesus macaque
Model NameMacaca mulatta (Rhesus Macaque)

Application Methodintra-tracheal instillation
Application Siteairway epithelium
Dosage10 uM DRI-NLS-Cy5, 40 uM S10 amphiphilic peptide
Injection Frequencyonce
Injection Raten/a
Injection Volume1 ml
Days post injection2 hours
Editor FormatDRI-NLS-Cy5 reporter peptide
Antidote Id
Antidote Description

Antibody RRIDRRID:AB_477585
Antibody Other IDT6793
Antibody Descriptionmouse monocolonal antibody against the acetylated α-tubulin, unconjugated, T6793, Sigma-Aldrich

Antibody RRIDRRID:AB_310759
Antibody Other ID07-623
Antibody Descriptionrabbit polyclonal antibody against club cell secretory protein, unconjugated, 07-623, EMD

Antibody RRIDRRID:AB_2717534
Antibody Other IDPA5-71680
Antibody Descriptionrabbit polyclonal antibody against the pulmonary-associated surfactant protein C (SPC), unconjugated, PA5-71680, Invitrogen

Antibody RRIDRRID:AB_2565050
Antibody Other ID905501.0
Antibody Descriptionrabbit polyclonal antibody to detect cytokeratin5+ basal cells, uncojugated, 905501, Biolegend

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of A•T basepairs converted to G•C
Mean 0.02 
1 0.0217