Experiment Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway Experiment Condition S10 and DRI-NLS-Cy5 (10 uM) Assay Description: Editing efficiency was assessed using Next Generation Sequencing of airway epithelial cells obtained by airway brushing in two airways of the airway tree Tissue Measured: lobar bronchus of left lung lower lobe Cell Type : bronchial epithelial cell Measurement Type: Editing Efficiency Record ID 15000003031 Editor Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide Vector Model Species Rhesus macaque Model Name Macaca mulatta (Rhesus Macaque) Application Method intra-tracheal instillation Application Site airway epithelium Dosage 10 uM DRI-NLS-Cy5, 40 uM S10 amphiphilic peptide Injection Frequency once Injection Rate n/a Injection Volume 1 ml Days post injection 2 hours Editor Format DRI-NLS-Cy5 reporter peptide Antidote Id Antidote Description Antibody RRID RRID:AB_477585 Antibody Other ID T6793 Antibody Description mouse monocolonal antibody against the acetylated α-tubulin, unconjugated, T6793, Sigma-Aldrich Antibody RRID RRID:AB_310759 Antibody Other ID 07-623 Antibody Description rabbit polyclonal antibody against club cell secretory protein, unconjugated, 07-623, EMD Antibody RRID RRID:AB_2717534 Antibody Other ID PA5-71680 Antibody Description rabbit polyclonal antibody against the pulmonary-associated surfactant protein C (SPC), unconjugated, PA5-71680, Invitrogen Antibody RRID RRID:AB_2565050 Antibody Other ID 905501.0 Antibody Description rabbit polyclonal antibody to detect cytokeratin5+ basal cells, uncojugated, 905501, Biolegend Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % of A•T basepairs converted to G•C Mean 0.01  1 0.0144