Experiment Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway Experiment Condition S10 and DRI-NLS-Cy5 (10 uM) Assay Description: Rhesus treated with Cy5+S10 peptide. Delivery efficiency was evaluated qualitatively, and determined by the presence of Cy5 positive nuclei in the airway epithelial cells in the various regions of the lungs. Tissue Measured: lower respiratory tract epithelium Cell Type : type II pneumocyte Measurement Type: Delivery Efficiency Record ID 15000003034 Editor Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide Vector Model Species Rhesus macaque Model Name Macaca mulatta (Rhesus Macaque) Sample Age 5 months Sample Sex Male Application Method intra-tracheal instillation Application Site airway epithelium Dosage 10 uM DRI-NLS-Cy5, 40 uM S10 amphiphilic peptide Injection Frequency once Injection Rate n/a Injection Volume 1 ml Days post injection 2 hours Editor Format DRI-NLS-Cy5 reporter peptide Antidote Id Antidote Description Antibody RRID RRID:AB_477585 Antibody Other ID T6793 Antibody Description mouse monocolonal antibody against the acetylated α-tubulin, unconjugated, T6793, Sigma-Aldrich Antibody RRID RRID:AB_310759 Antibody Other ID 07-623 Antibody Description rabbit polyclonal antibody against club cell secretory protein, unconjugated, 07-623, EMD Antibody RRID RRID:AB_2717534 Antibody Other ID PA5-71680 Antibody Description rabbit polyclonal antibody against the pulmonary-associated surfactant protein C (SPC), unconjugated, PA5-71680, Invitrogen Antibody RRID RRID:AB_2565050 Antibody Other ID 905501.0 Antibody Description rabbit polyclonal antibody to detect cytokeratin5+ basal cells, uncojugated, 905501, Biolegend Measured Values Samples Size: 1   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 1 of 1 present  1 present