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Experiment: Biomarker assays to evaluate toxicity and inflammation induced by AAVs in ES cell derived kidney organoids.

PI:   Ryuji Morizane, MD, PhD 

Description: Human kidney organoids generated from human ES are used to evaluate toxicity and inflammation induced by AAV2, AAV8, and AAV9 that express eGFP under CMV promoter. Organoids are exposed to AAVs at MOI 10^5 . MCP-1 and KIM-1 levels were measured using Luminex (Bioplex 200).
Biomarker Assay: KIM-1 and MCP-1 levels were measured using the Meso Scale Discovery (MSD) platform
Download: Submitted files
Filters ..
Delivery Systems
 AAV
Models
 Human kidney organoid (ESC-derived)
Sex
 Female
  Vectors
 AAV2/2CMVeGFP
 AAV2/8CMVeGFP
 AAV2/9CMVeGFP
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Results
Biomarker_assays_to_evaluate_toxicity_and_inflammation_induced_by_AAVs_in_ES_cell_derived_kidney_organoids.
Other Measurement
Record Id Condition Sex Model Delivery Vector Dosage Biomarker Detection MCP-1 (pg/mL) Biomarker Detection KIM-1 (pg/mL) Image
15000003424 Control Female Human kidney organoid (ESC-derived) control
15000003425 AAV2/2 Female Human kidney organoid (ESC-derived) AAV AAV2/2CMVeGFP MOI=10^5
15000003426 AAV2/8 Female Human kidney organoid (ESC-derived) AAV AAV2/8CMVeGFP MOI=10^5
15000003427 AAV2/9 Female Human kidney organoid (ESC-derived) AAV AAV2/9CMVeGFP MOI=10^5

Biomarker assays to evaluate toxicity and inflammation induced by AAVs in kidney organoids. H9 samples showing MCP-1 and KIM-1 levels using Luminex (Bio-plex 200). The infection rate was kept at 10^5 vg/cell.