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Delivery Systems
 Human kidney organoid (ESC-derived)
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Other Measurement
Record Id Condition Sex Model Delivery Vector Dosage Biomarker Detection MCP-1 (pg/mL) Biomarker Detection KIM-1 (pg/mL) Image
15000003424 Control Female Human kidney organoid (ESC-derived) control
15000003425 AAV2/2 Female Human kidney organoid (ESC-derived) AAV AAV2/2CMVeGFP MOI=10^5
15000003426 AAV2/8 Female Human kidney organoid (ESC-derived) AAV AAV2/8CMVeGFP MOI=10^5
15000003427 AAV2/9 Female Human kidney organoid (ESC-derived) AAV AAV2/9CMVeGFP MOI=10^5

Biomarker assays to evaluate toxicity and inflammation induced by AAVs in kidney organoids. H9 samples showing MCP-1 and KIM-1 levels using Luminex (Bio-plex 200). The infection rate was kept at 10^5 vg/cell.