| Delivery of saCas9 and gRNAs to nephron epithelia by AAV2 in kidney organoids. |
| Cas9+, mCherry+ in CDH1 Stained Cells |
| Calculated tranduction efficiceny of transgene delivery to nephron epithelia by counting Cas9+ mCherry+ fluorescent cells among CDH1+ stained loops of Henle/distal nephron cells |
|
| Delivery Efficiency |
| 15000003433 |
|
| SaCas9 (AAV-SaCas9) |
| AAV |
| Viral vector |
| DMD low gRNA-1; DMD low gRNA-2 |
| AAV2-gRNA; AAV2-SaCas9; |
|
| Human |
| Human kidney organoid (stem cell-derived) |
|
| virus |
|
| MOI=10^5 |
|
|
|
| 7 days |
| AAV vector encoded |
|
|
|
| RRID:AB_2336558 |
| B-1325-2 (Vector Labs) |
| Lotus tetragonolobus lectin (LTL) |
|
| RRID:AB_2722769 |
| ab205402 (Abcam) |
| Chicken anti-mCherry antibody |
|
|
| A01951 (GenScript) |
| mouse anti-saCas9 monoclonal antibody |
|
| Measured Values |
| Samples Size: | 5 |
| |
| Mean |
6.4 |
| 1 |
10 |
| 2 |
2.85714285714286 |
| 3 |
4.54545454545455 |
| 4 |
11.7647058823529 |
| 5 |
2.85714285714286 |