Experiment Delivery of saCas9 and gRNAs to nephron epithelia by AAV2 in kidney organoids. Experiment Condition Cas9+ mCherry- in CDH1 Stained Cells Assay Description: Calculated tranduction efficiceny of transgene delivery to nephron epithelia by counting Cas9+ mCherry- fluorescent cells among CDH1+ stained loops of Henle/distal nephron cells. Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003436 Editor SaCas9 (AAV-SaCas9) Delivery System AAV Delivery System Subtype Viral vector Guide DMD low gRNA-1; DMD low gRNA-2 Vector AAV2-gRNA; AAV2-SaCas9; Model Species Human Model Name Human kidney organoid (stem cell-derived) Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format AAV vector encoded Antidote Id Antidote Description Antibody RRID RRID:AB_2336558 Antibody Other ID B-1325-2 (Vector Labs) Antibody Description Lotus tetragonolobus lectin (LTL) Antibody RRID RRID:AB_2722769 Antibody Other ID ab205402 (Abcam) Antibody Description Chicken anti-mCherry antibody Antibody RRID Antibody Other ID A01951 (GenScript) Antibody Description mouse anti-saCas9 monoclonal antibody Measured Values Samples Size: 5   Replicate Result (Delivery Efficiency) Measurement Units: % of CDH+ cells Mean 1.68  1 2.5  2 0  3 0  4 5.88235294117647  5 0