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Editors
 SaCas9 (AAV-SaCas9)
Guide Target Locus
 DMD
Guides
 DMD low gRNA-2
Delivery Systems
 AAV
Models
 Human kidney organoid (stem cell-derived)
Vectors
 AAV2-gRNA
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Results

Evaluation_of_DNA_damage,_cellular_toxicity_and_inflammatory_markers_following_saCas9_and_gRNAs_delivery_by_AAV2_in_kidney_organoids.
Other Measurement
Record Id Condition Editor Model Delivery Target Locus Guide Vector Dosage Biomarker Detection %YH2AX+/LTL+ cells Biomarker Detection %YH2AX+/CHD1+ cells Biomarker Detection %YH2AX+/MEIS1/2/3+ cells Biomarker Detection %KIM1+/LTL+ cells Biomarker Detection KIM-1 (pg/mL) Biomarker Detection MCP-1 (pg/mL) Biomarker Detection NGAL (pg/mL) Biomarker Detection IL1-beta (pg/mL) Biomarker Detection IL-6 (pg/mL) Biomarker Detection IL-10 (pg/mL) Image
15000003441 no AAV control Human kidney organoid (stem cell-derived) MOI=10^5
15000003442 AAV2/2-GFP Human kidney organoid (stem cell-derived) AAV MOI=10^5
15000003443 AAV2/2-SaCas9 + AAV2/2-gRNA SaCas9 (AAV-SaCas9) Human kidney organoid (stem cell-derived) AAV DMD DMD low gRNA-2 AAV2-gRNA MOI=10^5

Toxicity and inflammatory responses to AAV2 and saCas9 genome editing. (C-F) Toxicity evaluation by immunohistochemistry. (G,H) Biomarker assays for toxicity and inflammation.