Experiment Imaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system Experiment Condition 2.4µg RNP-NC-tri(GalNAc) Assay Description: Calculated transfection efficiency by imaging fluorescent cells (Atto 550) compared to DAPI stained cells, and quantified using Image J. Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003503 Editor sNLS-SpCas9-sNLS Delivery System RNP-NC-tri(GalNAc) Delivery System Subtype Nanoparticle Guide Negative control gRNA Vector Model Species human Model Name Liver On Chip (LoC) Application Method Application Site Dosage 2.4µg RNP Injection Frequency Injection Rate Injection Volume Days post injection 24 hours Editor Format Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Delivery Efficiency) Measurement Units: Corrected total fluorescence (mean gray value) Mean 1  1 1.0