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ExperimentImaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system
Experiment Condition24µg RNP-NC Lipo 1:1
Assay Description: Calculated transfection efficiency by imaging fluorescent cells (Atto 550) compared to DAPI stained cells, and quantified using Image J.
Tissue Measured:
Measurement Type:Delivery Efficiency
Record ID15000003504

EditorsNLS-SpCas9-sNLS
Delivery SystemRNP-NC-no ligand with Lipofectamine 2000
Delivery System SubtypeNanoparticle
GuideNegative control gRNA
Vector

Model Specieshuman
Model NameLiver On Chip (LoC)

Application Methodchemical
Application Site
Dosage24µg RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection24 hours
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Delivery Efficiency)
Measurement Units: Corrected total fluorescence (mean gray value)
Mean
1 1.0