Experiment Validating Traffic Light Reporter 2 (TLR2) Mouse Model in Homozygous Blastocysts using SpCas9 Experiment Condition Guide 26-3 Assay Description: Reporter activation by NHEJ is calculated as the percent of blasts that express tagRFP out of the total blasts imaged. Approximately one third of NHEJ events are detected. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000005233 Editor Alt-R® S.p. Cas9 Nuclease V3 Delivery System Delivery System Subtype Guide R26-3 Vector Model Species Mouse Model Name TLR-2 homozygous blastocyst Application Method Electroporation Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection 96 hours Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: # blasts assessed by sequencing Mean # blasts analyzed  1 88 blasts  Measurement Units: # blasts assessed for RFP activation Mean # blasts analyzed  1 193 blasts  Measurement Units: % of blasts with RFP activation Mean 53.9  1 53.9  Measurement Units: % of blasts with edits Mean 100  1 100.0