g-loxPbot_C12a | SCGE Toolkit

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Summary Related Editors Projects & Experiments

SCGE ID	10000000079
Lab ID	g-loxPbot_C12a
Source	IDT
Target Locus	Ai9/Ai14 reporter transgene (within loxP)
Species	transgene
Description	This sgRNA targets the Ai9 and related transgenes at two sites
Guide Compatibility	AsCas12a

Target Sequence	GTATAATGTATGCTATACGAA
Target Sequence + PAM	GTATAATGTATGCTATACGAAGTTA

Spacer Sequence	GUAUAAUGUAUGCUAUACGAA
Spacer Length	21
Modifications	none

AsCas12a (IDT and Feldan Therapeutics)

Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI

This Guide: g-loxPbot_C12a is being used ...

Project	Initiative	Contact PI
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia	Delivery Systems Initiative	Paul B McCray, Jr, MD (University of Iowa)