Experiment: Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
PI: Erik J Sontheimer, PhD| Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Neuro2A cells. Editing activity was determined by Sanger sequencing |
| Editing Assay: |
- Calculated editing efficiency by PCR amplification and sanger sequencing analysis. |
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Results |
| Editing Efficiency | ||||||||
|---|---|---|---|---|---|---|---|---|
| Condition | Sex | Editor | Model | Target Locus | Guide | Dosage | % of Lesion | Image |
| STS204 (DNMT1) (5 pmol RNP) | Male | SpyCas9-3xNLS | Neuro 2A | Dnmt1 | STS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1) | 5 pmol RNP | ||
| STS205 (BACE1) (5 pmol RNP) | Male | SpyCas9-3xNLS | Neuro 2A | Bace1 | STS205 (BACE1; Sp_t41:Sp_c20_Bace1) | 5 pmol RNP | ||
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