R2-modified | SCGE Toolkit

SCGE ID	10000000224
Lab ID	R2-modified
Source	Vector encoded
Target Locus	Ai9/Ai14 reporter transgene
Species	transgene
Description	This gRNA targets the Ai9 and related transgenes
Guide Compatibility	SauCas9

Target Sequence	CGACCTGCAGCCCAAGCTAGA
Target Sequence + PAM	CGACCTGCAGCCCAAGCTAGATCGAAT

Full Guide Sequence	CGACCUGCAGCCCAAGCUAGAGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer Sequence	CGACCUGCAGCCCAAGCUAGA
Spacer Length	21
Modifications	GUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification

This Guide: R2-modified is being used ...

Project	Initiative	Contact PI
Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro	Delivery Systems Initiative	Benjamin E Deverman, PhD (Broad Institute)

Guide: R2-modified