SCGE ID10000000224
Lab IDR2-modified
SourceVector encoded
Target LocusAi9/Ai14 reporter transgene
Speciestransgene
DescriptionThis gRNA targets the Ai9 and related transgenes
Guide CompatibilitySauCas9

Target SequenceCGACCTGCAGCCCAAGCTAGA
Target Sequence + PAMCGACCTGCAGCCCAAGCTAGATCGAAT

Full Guide SequenceCGACCUGCAGCCCAAGCUAGAGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer SequenceCGACCUGCAGCCCAAGCUAGA
Spacer Length21
ModificationsGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification

  • R2-unmodified

  • SaCas9



  • This Guide: R2-modified is being used ...
    Project Initiative Contact PI
    Delivery Systems Benjamin E Deverman, PhD
    (Broad Institute)
    NIH Report