Experiment: Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
PI: Benjamin E Deverman, PhD
Description: 3e11 vg/mouse of AAV-BI28:GFAP-SaCas9-WPRE-pA and 3e11 vg/mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 mice. Editing was assessed in brain sections 4 weeks later.
Editing Assay:
Editing efficiency assessed by quantifying tdTomato positive area above threshold
Parent Project: Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System
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Other experiments in this project: 1
Download: Submitted files
Organ System Overview
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