L2-modified | SCGE Toolkit

SCGE ID	10000000226
Lab ID	L2-modified
Source	Vector encoded
Target Locus	Ai9/Ai14 reporter transgene
Species	transgene
Description	This gRNA targets the Ai9 and related transgenes
Guide Compatibility	SauCas9

Target Sequence	TCTAGAGGATCTGCGACTCTA
Target Sequence + PAM	TCTAGAGGATCTGCGACTCTAGAGGAT

Full Guide Sequence	UCUAGAGGAUCUGCGACUCUAGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer Sequence	UCUAGAGGAUCUGCGACUCUA
Spacer Length	21
Modifications	GUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold region modification

This Guide: L2-modified is being used ...

Project	Initiative	Contact PI
Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Experiments Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro	Delivery Systems	Benjamin E Deverman, PhD (Broad Institute)
BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice Experiments Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Jason D Heaney, PhD (Baylor College of Medicine)

Guide: L2-modified