Cas12j-GFP12 (CasΦ-3) | SCGE Toolkit

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Summary Related Editors Projects & Experiments

SCGE ID	10000000282
Lab ID	Cas12j-GFP12 (CasΦ-3)
Target Locus	eGFP
Species	transgene
Description	Guide targeting eGFP compatible with CasΦ-3
Guide Compatibility	CasΦ-3

Target Sequence	ACCTTGATGCCGTTCTTCTG
Target Sequence + PAM	GTTCACCTTGATGCCGTTCTTCTG

Full Guide Sequence	UGCCCAGUACGCUGGGACACCUUGAUGCCGUUCUUCUG
Spacer Sequence	ACCUUGAUGCCGUUCUUCUG
Spacer Length	20
Modifications	none

Publication Title
CRISPR-CasΦ from huge phages is a hypercompact genome editor. NCBI

This Guide: Cas12j-GFP12 (CasΦ-3) is being used ...

Project	Initiative	Contact PI
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Experiments Testing newly discovered Biggie Phage editors in human cells	Genome Editors Initiative	Jennifer A Doudna, PhD Jillian Banfield, PhD (University of California, Berkeley)