Genome Editor: Casϕ-3
Summary
| SCGE ID | 11000000106 |
| Symbol | CasΦ-3 |
| Description | Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies |
| Species | Biggie Phage |
| Type | Cas Nuclease |
| Subtype | Cas12j |
| Alias | Cas12j-3 |
| Activity | Double strand cleavage |
| Substrate | double stranded DNA |
| DSB Cleavage Type | Staggered |
| PAM | 5' VTTN |
| Fusion | 2X C-terminal SV40NLS, C-terminal 2xFLAG tag |
| Source | Jennifer Doudna Lab |
Protein Sequence
MEKEITELTKIRREFPNKKFSSTDMKKAGKLLKAEGPDAVRDFLNSCQEIIGDFKPPVKTNIVSISRPFEEWPVSMVGR IQEYYFSLTKEELESVHPGTSSEDHKSFFNITGLSNYNYTSVQGLNLIFKNAKAIYDGTLVKANNKNKKLEKKFNEINH RSLEGLPIITPDFEEPFDENGHLNNPPGINRNIYGYQGCAAKVFVPSKHKMVSLPKEYEGYNRDPNLSLAGFRNRLEIP GEPGHVPWFQRMDIPEGQIGHVNKIQRFNFVHGKNSGKVKFSDKTGRVKRYHHSKYKDATKPYKFLEESKKVSALDSIL IITIGDDWVVFDIRGLYRNVFYRELAQKGLTAVQLLDLFTGDPVIDPKKGVVTFSYKEGVVPVFSQKIVPRFKSRDTLE LTSQGPVALLSVDLGQNEPVAARVCSLKNINDKITLDNSCRISFLDDYKKQIKDYRDSLDELEIKIRLEAINSLETNQQ EIRDLDVFSADRAKANTVDMFDIDPNLISWDSMSDARVSTQISDLYLKNGGDESRVYFEINNKRIKRSDYNISQLVRPK SDSTRKNLNDSIWKLKRTSEEYLKLSKRKLELSRAVVNYTIRQSKLLSGINDIVIILEDLDVKKKFNGRGIRDIGWDNF SSRKENRWFIPAFHKAFSELSSNRGLCVIEVNPAWTSATCPDCGFCSKENRDGINFTCRKCGVSYHADIDVATLNIARV VLGKPMSGPADRERLGDTKKPRVARSRKTMKRKDISNSTVEAMVTAGSGPKKKRKVEDPKKKRKVSLGSGSDYKDDDDK YKDDDDK |
Associated Publications |
| Publication Title |
|---|
| CRISPR-CasΦ from huge phages is a hypercompact genome editor. NCBI |
Projects & Experiments
This Genome Editor: Casϕ-3 is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Genome Editors Initiative |
Jennifer A Doudna, PhD
Jillian Banfield, PhD
(University of California, Berkeley) |
|