Deverman_AAV Production and Administration Protocol

SCGE ID	21000000010
Title	Deverman_AAV Production and Administration Protocol
Description	Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.
Tier	4
File Download	Deverman_AAV_production_and_administration_Nat Protocols-Challis_et_al_2019.pdf
Additional Information	https://pubmed.ncbi.nlm.nih.gov/30626963/
Keywords	mouse, mice, intravenous, systemic delivery, systemic, delivery, adeno-associated virus, adeno-associated viral, AAV, virus, viral, AAV capsid, central nervous system, CNS, peripheral nervous system, PNS, immunofluorescence, IF, fluorescence, fluorescently, fluoresent, imaging

This Protocol: Deverman_AAV Production and Administration Protocol is being used ...

Project	Initiative	Contact PI
Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Experiments Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro	Delivery Systems Initiative	Benjamin E Deverman, PhD (null)

Protocol: Deverman_AAV Production and Administration Protocol