Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentGene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
Experiment ConditionS10, Cas12a, HPRT
Assay Description: Calculated editing efficiency mean of 3 biological replicates by sanger sequencing.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000539

EditorAsCas12a (Feldan Therapeutics)
Delivery SystemS10
Delivery System SubtypeAmphiphilic peptide
Guideg-38330_C12a
Vector

Model SpeciesHuman
Model NamePrimary Airway Epithelia

Application MethodIn Media
Application Site
Dosage[RNP]: 2.5 µM; [gRNA]: 2.0 μM; [Peptide]: 40uM
Injection Frequency
Injection Rate
Injection Volume
Days post injection72 hours
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % indel
Mean 22.00 
1 22.0 
  

Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI