Experiment Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Experiment Condition L2+R2, modified scaffold Assay Description: Editing efficiency was assessed by flow cytometry for tdTomato expression in Ai9 mouse embryonic fibroblasts after transfection with a mixture of plasmids expressing SaCas9 and the indicated U6-gRNAs Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000574 Editor SaCas9 Delivery System Delivery System Subtype Guide R2-modified; L2-modified Vector AAV-CMV-SaCas9-U6-modified scaffold-Sa-L2; AAV-CMV-SaCas9-U6-modified scaffold-Sa-R2; Model Species Mouse Model Name Ai9 Immortalized Fibroblasts Application Method transient transfection Application Site Dosage Injection Frequency Injection Rate Injection Volume Days post injection Editor Format plasmid Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % tdTomato+ cells Mean 10.13  1 16.49  2 4.27  3 9.62