Experiment Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Experiment Condition Sp_c20_mTmG/Sp_t2 Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000000701 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS159 (mTmG; Sp_t2:Sp_c20_mTmG) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Sample Age 7-8 weeks Application Method Intra-Striatum Application Site brain Dosage 100 µM RNP Injection Frequency once Injection Rate 500 nl/min Injection Volume 2-3 microliters Days post injection 1 or 2 weeks Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Antibody RRID RRID:AB_2536526 Antibody Other ID Thermo Fisher Scientific Cat# G10362 Antibody Description GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362 Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 4 of 4 present  1 present  2 present  3 present  4 present