Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition scAAV5-Cre-GFP Pos Control, Ai9 - SauSpyCas9, female Assay Description: Fluorescent imaging for tdTomato+ cells Tissue Measured: epithelium of bronchiole Cell Type : respiratory epithelial cell Measurement Type: Editing Efficiency Record ID 15000001083 Editor Cre recombinase Delivery System Delivery System Subtype Guide Vector scAAV5-Cre-GFP; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Sample Age 8 weeks Sample Sex Female Application Method intratracheal intubation Application Site lung airway epithelium Dosage 1.7E11 viral genomes Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format virus in sterile saline Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 2 of 2 Significant red cells  1 Significant red cells  2 Significant red cells