Experiment Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center Experiment Condition mTmG-C20/T2 Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: striatum Measurement Type: Editing Efficiency Record ID 15000001497 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS159 (mTmG; Sp_t2:Sp_c20_mTmG) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Sample Sex Male Application Method Application Site brain Dosage Injection Frequency once Injection Rate Injection Volume 3 microliters, bilateral Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Antibody RRID RRID:AB_1196615 Antibody Other ID AB_1196615 Antibody Description GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean Present  1 Present  2 Present  3 Present  4 Present

GFP staining of mTmG mouse injected with mTmG-C20/T2 guide none provided