Experiment On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver Experiment Condition OT3 (A4)-AAV Assay Description: adenine base editing at target Pcsk9 site in liver vs. other mouse organs (heart, kidney, lung, muscle, spleen) Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000001550 Editor TadA-8e V106W Delivery System Delivery System Subtype Guide Vector AAV-Pcsk9; Model Species Mouse Model Name C57BL/6J Application Method intravenous, retro-orbital Application Site liver Dosage 1E11 vg Injection Frequency once Injection Rate Injection Volume 120 microliters Days post injection 1 week Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 5   Replicate Result (Editing Efficiency) Measurement Units: % of total sequencing reads with AT converted to GC Mean 0.01  1 0.008527  2 0.013148  3 0.019002  4 0.016496  5 0.004573