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Tissues
Liver and Biliary
  • Liver  (TARGET)
Editors
 TadA-8e V106W
Delivery Systems
 eVLP
Models
 C57BL/6J mouse
Sex
 Male
Vectors
 AAV-Pcsk9
Select experimental variable to highlight records on the chart: 
 

Note: Hover over the bars to view additional information

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Results

On-target_editing_compared_to_14_circle-seq_nominated_off-target_sites_of_adenine_base_editor_delivered_by_BE-eVLP_vs_AAV_in_the_C57BL/6_liver
Editing Efficiency
Record Id Condition Tissue Sex Editor Model Delivery Vector Dosage Injection Frequency Editing Efficiency % of total sequencing reads with AT converted to GC Image
15000001532 OnTarget-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001533 OT1 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001534 OT2 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001535 OT3 (A4)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001536 OT4 (A4)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001537 OT5 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001538 OT6 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001539 OT7 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001540 OT8 (A4)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001541 OT9 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001542 OT10 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001543 OT11 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001544 OT12 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001545 OT13 (A6)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001546 OT14 (A4)-BE-eVLP liver (TARGET) Male TadA-8e V106W C57BL/6J mouse eVLP 7E11 eVLPs 1x
15000001547 OnTarget-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001548 OT1 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001549 OT2 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001550 OT3 (A4)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001551 OT4 (A4)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001552 OT5 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001553 OT6 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001554 OT7 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001555 OT8 (A4)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001556 OT9 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001557 OT10 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001558 OT11 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001559 OT12 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001560 OT13 (A6)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001561 OT14 (A4)-AAV liver (TARGET) Male TadA-8e V106W C57BL/6J mouse AAV-Pcsk9 1E11 vg 1x
15000001562 OT1 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001563 OT2 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001564 OT3 (A4)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001565 OT4 (A4)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001566 OT5 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001567 OT6 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001568 OT7 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001569 OT8 (A4)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001570 OT9 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001571 OT10 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001572 OT11 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001573 OT12 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001574 OT13 (A6)-Untreated liver (TARGET) Male C57BL/6J mouse
15000001575 OT14 (A4)-Untreated liver (TARGET) Male C57BL/6J mouse

Title Description File Download SCGE ID
Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis.pdf 21000000033

Off-target editing with BE-eVLP versus AAV treatment

DNA sequencing reads containing A-T to G-C

mutations within protospacer positions 4-10 for the 14 CIRCLE-

seq-nominated off-target loci from the livers of BE-eVLP-treated,

AAV-treated, and untreated mice. Data are shown as individual

data points and mean + =/- SEM for n=4 mice (BE-VLP), n=5 mice

(AAV), or n=3 mice (untreated). vg, viral genomes.