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Filters ..
Tissues
Respiratory
  • Epithelium of bronchus (club cell)  (TARGET)
  • Epithelium of bronchus (ciliated cell of the bronchus)  (TARGET)
  Cell Types
 ciliated cell of the bronchus
 club cell
Editors
 SpCas9
Guide Target Locus
 Ai9/Ai14 reporter transgene
  Guides
 sgAi9L
 sgAi9R
Models
 Ai9 mouse
Sex
 Female
  Vectors
 AAV.pU1a-SpCas9
 H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)
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Results

Testing_AAV5_for_activation_of_tdTomato_in_mouse_airway_club_and_ciliated_cells
Editing Efficiency
Record Id Condition Tissue Cell Type Sex Editor Model Target Locus Guide Vector Dosage Injection Frequency Editing Efficiency % cells Image
15000001638 AAV-SpCas9 epithelium of bronchus (TARGET) club cell Female SpCas9 Ai9 mouse AAV.pU1a-SpCas9 6e10 gc/each AAV per mouse by intratracheal injection once
15000001639 AAV-SpCas9 epithelium of bronchus (TARGET) ciliated cell of the bronchus Female SpCas9 Ai9 mouse AAV.pU1a-SpCas9 6e10 gc/each AAV per mouse by intratracheal injection once
15000001640 Dual AAV epithelium of bronchus (TARGET) club cell Female SpCas9 Ai9 mouse Ai9/Ai14 reporter transgene sgAi9RsgAi9L AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 6e10 gc/each AAV per mouse by intratracheal injection once
15000001641 Dual AAV epithelium of bronchus (TARGET) ciliated cell of the bronchus Female SpCas9 Ai9 mouse Ai9/Ai14 reporter transgene sgAi9RsgAi9L AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 6e10 gc/each AAV per mouse by intratracheal injection once

AAV5 mediates genome editing in club and ciliated cells.