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Tissues
Respiratory
  • Epithelium of main bronchus (epithelial cell of lung)  (TARGET)
Cell Types
 epithelial cell of lung
Editors
 SpCas9
Guide Target Locus
 Ai9/Ai14 reporter transgene
  Guides
 sgAi9L
 sgAi9R
Models
 Ai9 mouse
Sex
 Female
  Vectors
 AAV.pU1a-SpCas9
 H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)
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Results

Testing_AAV5_for_activation_of_tdTomato_in_mouse_airway_
Editing Efficiency Delivery Efficiency
Record Id Condition Tissue Cell Type Sex Editor Model Target Locus Guide Vector Dosage Injection Frequency Editing Efficiency % cells Delivery Efficiency % cells Image
15000001632 Saline epithelium of main bronchus (TARGET) epithelial cell of lung Female Ai9 mouse once
15000001633 Saline epithelium of main bronchus (TARGET) epithelial cell of lung Female Ai9 mouse once
15000001634 AAV-SpCas9 epithelium of main bronchus (TARGET) epithelial cell of lung Female SpCas9 Ai9 mouse AAV.pU1a-SpCas9 6e10 gc/each AAV per mouse by intratracheal injection once
15000001635 AAV-SpCas9 epithelium of main bronchus (TARGET) epithelial cell of lung Female SpCas9 Ai9 mouse AAV.pU1a-SpCas9 6e10 gc/each AAV per mouse by intratracheal injection once
15000001636 Dual AAV epithelium of main bronchus (TARGET) epithelial cell of lung Female SpCas9 Ai9 mouse Ai9/Ai14 reporter transgene sgAi9RsgAi9L AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 6e10 gc/each AAV per mouse by intratracheal injection once
15000001637 Dual AAV epithelium of main bronchus (TARGET) epithelial cell of lung Female SpCas9 Ai9 mouse Ai9/Ai14 reporter transgene sgAi9RsgAi9L AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 6e10 gc/each AAV per mouse by intratracheal injection once

Dual AAV5 delivery of spCas9 and sgRNAs supports genome editing in mouse lung.