Experiment: Testing AAV5 for activation of tdTomato in mouse airway club and ciliated cells
PI: Guang-Ping Gao, PhD
Description: AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Gene editing quantified by tdTomato activation and cell specific markers for club and ciliated cell types.
Editing Assay:
Editing efficiency calculated by counting cells double positive for tdTomato and CCSP (a marker of club/clara cells) and acetylated tubulin (a marker of ciliated cells) compared to DAPI stained cells. Analyzed 5 sections per mouse.
Parent Project: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
-
Other experiments in this project: 2
Download: Submitted files
Analyze Data Sets Available for this Experiment
Respiratory
| Epithelium of bronchus (club cell) (TARGET) |
| Epithelium of bronchus (ciliated cell of the bronchus) (TARGET) |
Organ System Overview
|