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Editors
 Alt-R® S.p. Cas9 Nuclease V3
Guide Target Locus
 GER12 (CAG-Venus*,-Katushka2S,-luc)
  Guides
 GER12-g1-FWD
 GER12-g2-FWD
Models
 GER12 Heterozygous Blastocyst
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Results

Validating_Gene_Editing_Reporter_12_(GER12)_Mouse_Model_in_Heterozygous_Blastocysts
Editing Efficiency
Record Id Condition Editor Model Target Locus Guide Editing Efficiency Signal Editing Efficiency % of blasts with RFP activation Editing Efficiency % of blasts with edits Image
15000003589 GER12-g1-FWD Alt-R® S.p. Cas9 Nuclease V3 GER12 Heterozygous Blastocyst GER12 (CAG-Venus*,-Katushka2S,-luc) GER12-g1-FWD
15000003590 GER12-g2-FWD Alt-R® S.p. Cas9 Nuclease V3 GER12 Heterozygous Blastocyst GER12 (CAG-Venus*,-Katushka2S,-luc) GER12-g2-FWD

Validation of Gene Editing Reporter 12 (GER12) Mouse Model in Heterozygous Blastocysts

Zygotes were electroporated with Cas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.