Rhesus macaque CCR5 gRNA screening using ABE8e-Cas9 | SCGE Toolkit

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Results

Rhesus_macaque_CCR5_gRNA_screening_using_ABE8e-Cas9
							Editing Efficiency
Record Id	Condition	Editor	Model	Delivery	Target Locus	Guide	Dosage	Editing Efficiency % of reads	Image
15000002939	ABE8e-Cas9 Guide 1	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 1 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	32.4;
15000002940	ABE8e-Cas9 Guide 2	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 2 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	0.0;
15000002941	ABE8e-Cas9 Guide 3	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 3 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	49.0;
15000002942	ABE8e-Cas9 Guide 4	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 4 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	39.3;
15000002943	ABE8e-Cas9 Guide 5	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 5 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	33.3;
15000002944	ABE8e-Cas9 Guide 6	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 6 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	49.0;
15000002945	ABE8e-Cas9 Guide 7	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 7 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	46.6;
15000002946	ABE8e-Cas9 Guide 8	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 8 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	54.7;
15000002947	ABE8e-Cas9 Guide 9	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 9 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	47.9;
15000002948	ABE8e-Cas9 Guide 10	ABE8e	Primary skin fibroblasts (Rhesus Macaque)	SE Cell Line 4D-Nucleofector™ X Kit S	CCR5	ABE8e-Cas9 Guide 10 CCR5 Rhesus	26 µM gRNA and ABE8e-Cas9 11 µM in a total volume of 10 ml RNP	44.5;