Experiment: Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to rhesus monkey airway epithelial cells cultured at the air liquid interface targeting CCR5 locus.
PI: Paul B McCray, Jr, MD Alice F Tarantal, PhD David Guay, PhD David R Liu, PhD
Description: Delivery of ABE8e-Cas9 RNP using Shuttle peptides candidates to rhesus monkey airway epithelial cells cultured at the air liquid interface targeting CCR5 locus. Editing efficiency was assessed using Next Generation Sequencing.
Editing Assay:
Editing efficiency was assessed using Next Generation Sequencing
Parent Project: Base Editing in Rhesus Airway Epithelial
-
Other experiments in this project: 6
- Delivery of Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting CFTR locus
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas9
- Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas12a
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting B2M locus
- Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Download: Submitted files
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
| Editing Efficiency | |||||||
|---|---|---|---|---|---|---|---|
| Condition | Editor | Model | Delivery | Target Locus | Guide | % of reads | Image |
| Mock | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | CCR5 | gRNA #8 | |||
| FSD63d1 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD63D1 | CCR5 | gRNA #8 | ||
| FSD262 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD262 | CCR5 | gRNA #8 | ||
| FSD95 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD95 | CCR5 | gRNA #8 | ||
| FSD237 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD237 | CCR5 | gRNA #8 | ||
| S10 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | S10 | CCR5 | gRNA #8 | ||
| FSD321 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD321 | CCR5 | gRNA #8 | ||
| FSD315 | ABE8e-Cas9 | Primary Airway Epithelia (Rhesus Macaque) | FSD315 | CCR5 | gRNA #8 | ||
× Close Overview
