Experiment: Delivery of Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting CFTR locus
PI: Paul B McCray, Jr, MD Alice F Tarantal, PhD David Guay, PhD David R Liu, PhD
Description: Delivery of Cas9 RNP using Shuttle peptides candidates to human airway epithelial cells cultured at the air liquid interface targeting CFTR locus. Editing efficiency was assessed using NGS.
Editing Assay:
Editing efficiency was assessed using Next Generation Sequencing
Parent Project: Base Editing in Rhesus Airway Epithelial
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Other experiments in this project: 6
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas9
- Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas12a
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting B2M locus
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to rhesus monkey airway epithelial cells cultured at the air liquid interface targeting CCR5 locus.
- Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Download: Submitted files
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