Experiment: Rhesus macaque CCR5 gRNA screening using ABE8e-Cas12a
PI: Paul B McCray, Jr, MD Alice F Tarantal, PhD David Guay, PhD David R Liu, PhD
Description: Electroporation of plasmids containing ABE8e-Cas12a with 11 different gRNAs targeting CCR5 into rhesus primary skin fibroblasts. Editing efficiency was assessed using high throughput DNA sequencing.
Editing Assay:
Editing efficiency was assessed using Next Generation Sequencing
Parent Project: Base Editing in Rhesus Airway Epithelial
-
Other experiments in this project: 6
- Delivery of Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting CFTR locus
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas9
- Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting B2M locus
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to rhesus monkey airway epithelial cells cultured at the air liquid interface targeting CCR5 locus.
- Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Download: Submitted files
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
× Close Overview
