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24µg RNP-NC Lipo 1:1 | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
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Experiment Record Detail
Experiment
Quantification of transfection efficiency by flow cytometry with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on liver-on-chip model system
Experiment Condition
24µg RNP-NC Lipo 1:1
Assay Description:
Flow cytometry
Tissue Measured:
Measurement Type:
Delivery Efficiency
Record ID
15000003511
Editor
sNLS-SpCas9-sNLS
Delivery System
RNP-NC-no ligand with Lipofectamine 2000
Delivery System Subtype
Nanoparticle
Guide
Negative control gRNA
Vector
Model Species
human
Model Name
Liver On Chip (LoC)
Application Method
chemical
Application Site
Dosage
24µg RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection
Editor Format
Antidote Id
Antidote Description
Measured Values
Samples Size:
1
Replicate
Result (Delivery Efficiency)
Measurement Units:
% uptake
Mean
29.6 
1
29.6