Experiment Validating Traffic Light Reporter 2 (TLR-2) Mouse Model via Germline Editing Experiment Condition Guide 26-2 Assay Description: Detection of tagRFP fluorescence in tissues collected from adult TLR-2 mice edited through the germline (N1 progeny). Tissue Measured: diaphragm Measurement Type: Editing Efficiency Record ID 15000005239 Editor Alt-R™ S.p. Cas9 Nuclease V3 Delivery System Delivery System Subtype Guide R26-2 Vector Model Species Mouse Model Name Traffic Light Reporter 2 mouse Application Method Electroporation Application Site Dosage Injection Frequency once Injection Rate Injection Volume Days post injection N1 generation Editor Format RNP Antidote Id Antidote Description Antibody RRID RRID:AB_10563941 Antibody Other ID Invitrogen, R10367 Antibody Description Polyclonal rabbit anti-RFP antibody Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 6 of 6 present  1 present  2 present  3 present  4 present  5 present  6 present