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Experiment: Validating Traffic Light Reporter 2 (TLR-2) Mouse Model via Germline Editing

PI:   Stephen A Murray, PhD 

Description: Heterozygous embryos containing the traffic light reporter 2 (TLR-2) reporter at the Rosa26 locus were evaluated for activation potential following electroporation of two guide/RNP combinations and transfer to pseudo-pregnant hosts for development to term. Offspring (founders) with activating edits were mated to wild-type females and adult tissues from progeny were assessed for tagRFP fluorescence.
Editing Assay: Detection of tagRFP fluorescence in tissues collected from adult TLR-2 mice edited through the germline (N1 progeny).
Download: Submitted files
Filters ..
  Tissues
Reproductive
  • Uterus
  • Ovary
  • Epididymis
  • Testis
Renal/Urinary
  • Urinary bladder
  • Kidney
Endocrine
  • Pancreas
Haemolymphoid
  • Lymph node
  • Spleen
  • Thymus
Gastrointestinal
  • Large intestine
  • Small intestine
  • Stomach
Liver and Biliary
  • Liver
Respiratory
  • Lung
Cardiovascular
  • Heart
Musculoskeletal
  • Diaphragm
  • Gastrocnemius
Integumentary
  • Skin of body
Nervous
  • Brain
Sensory
  • Eye
Editors
 Alt-R® S.p. Cas9 Nuclease V3
Guide Target Locus
 Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn
  Guides
 R26-2
 R26-52
Models
 TLR-2 mouse

Results
Validating_Traffic_Light_Reporter_2_(TLR-2)_Mouse_Model_via_Germline_Editing
Editing Efficiency
Condition
Tissue
Editor
Model
Target Locus
Guide
Injection Frequency
Editing Efficiency Signal
Image
15000005237 Guide 26-2 urinary bladder Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005238 Guide 26-2 brain Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005239 Guide 26-2 diaphragm Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005240 Guide 26-2 eye Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005241 Guide 26-2 gastrocnemius Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005242 Guide 26-2 heart Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005243 Guide 26-2 kidney Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005244 Guide 26-2 large intestine Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005245 Guide 26-2 liver Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005246 Guide 26-2 lung Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005247 Guide 26-2 lymph node Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005248 Guide 26-2 pancreas Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005249 Guide 26-2 skin of body Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005250 Guide 26-2 small intestine Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005251 Guide 26-2 spleen Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005252 Guide 26-2 stomach Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005253 Guide 26-2 thymus Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005254 Guide 26-2 uterus Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005255 Guide 26-2 ovary Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005256 Guide 26-2 epididymis Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005257 Guide 26-2 testis Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005258 Guide 26-52 urinary bladder Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005259 Guide 26-52 brain Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005260 Guide 26-52 diaphragm Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005261 Guide 26-52 eye Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005262 Guide 26-52 gastrocnemius Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005263 Guide 26-52 heart Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005264 Guide 26-52 kidney Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005265 Guide 26-52 large intestine Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005266 Guide 26-52 liver Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005267 Guide 26-52 lung Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005268 Guide 26-52 lymph node Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005269 Guide 26-52 pancreas Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005270 Guide 26-52 skin of body Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005271 Guide 26-52 small intestine Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005272 Guide 26-52 spleen Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005273 Guide 26-52 stomach Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005274 Guide 26-52 thymus Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005275 Guide 26-52 uterus Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005276 Guide 26-52 ovary Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005277 Guide 26-52 epididymis Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005278 Guide 26-52 testis Alt-R® S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once