Experiment Validating Traffic Light Reporter 2 (TLR-2) Mouse Model via Germline Editing Experiment Condition Guide 26-2 Assay Description: Detection of tagRFP fluorescence in tissues collected from adult TLR-2 mice edited through the germline (N1 progeny). Tissue Measured: thymus Measurement Type: Editing Efficiency Record ID 15000005253 Editor Alt-R™ S.p. Cas9 Nuclease V3 Delivery System Delivery System Subtype Guide R26-2 Vector Model Species Mouse Model Name Traffic Light Reporter 2 mouse Sample Age adult N1s Application Method Electroporation Application Site Dosage Injection Frequency once Injection Rate Injection Volume Days post injection N1 generation Editor Format RNP Antidote Id Antidote Description Antibody RRID RRID:AB_10563941 Antibody Other ID Invitrogen, R10367 Antibody Description Polyclonal rabbit anti-RFP antibody Measured Values Samples Size: 0   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 6 of 6 present  1 present  2 present  3 present  4 present  5 present  6 present