L1-modified | SCGE Toolkit>

SCGE ID	10000000221
Lab ID	L1-modified
Source	Vector encoded
Target Locus	Ai9/Ai14 reporter transgene
Species	Transgene
Description	This gRNA targets the Ai9 and related transgenes
Guide Compatibility	SauCas9

Target Sequence	CCTCTAGAGTCGCAGATCCTC
Target Sequence + PAM	CCTCTAGAGTCGCAGATCCTCTAGAGT

Full Guide Sequence	CCUCUAGAGUCGCAGAUCCUCGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer Sequence	CCUCUAGAGUCGCAGAUCCUC
Spacer Length	21
Modifications	GUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold region modification

This Guide: L1-modified is being used

Project	Initiative	Contact PI
Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro	Delivery Systems Initiative	Benjamin E Deverman, PhD (Broad Institute)
BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice Experiments Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain	Animal Reporter and Testing Center Initiative	Jason D Heaney, PhD (Baylor College of Medicine)

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