Guide: STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG)
Summary
SCGE ID | 10000000234 |
Lab ID | STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG) |
Source | in house production |
Target Locus | mTmG reporter transgene |
Species | transgene |
Description | This sgRNA targets the mTmG transgene |
Guide Compatibility | SpyCas9 |
Target Sequence | CGAAGTTATATTAAGGGTTC |
Target Sequence + PAM | CGAAGTTATATTAAGGGTTCGGG |
Full Guide Sequence | Sp_t2-PS: AAUCGAAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUAUGCA Sp_c0_mTmG: CGAAGUUAUAUUAAGGGUUCGUUUUAGAGCUAUGCU |
Spacer Sequence | CGAAGUUAUAUUAAGGGUUC |
Spacer Length | 20 |
Modifications | Sp_t2-PS: (mA)#(mA)#(mU)#(mC)#(mG)#(mA)#(mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)#(mU)#(mA)#(mU)#(mG)#(mC)#(mA) Sp_c0_mTmG: (rC)#(rG)#(rA)#(rA)(rG)(rU)(rU)(rA)(rU)(rA)(rU)(rU)(rA)(rA)(rG)(rG)(rG)(rU)(rU)(rC)(rG)(rU)(rU)(rU)(rU)(rA)(rG)(rA)(rG)(rC)(rU)(rA)(rU)#(rG)#(rC)#(rU) |
Repeat Sequence | GUUUUAGAGCUAUGCU |
Anit-Repeat Sequence | AGCAUAGCAAGUUAAAAU |
Stemloop 1 Sequence | AAGGCUAGUCCGU |
Stemloop 2 Sequence | AACUUGAAAAAGUG |
Stemloop 3 Sequence | GCACCGAGUCGGUGC |
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