Experiment Testing newly discovered Biggie Phage editors in human cells Experiment Condition Cas12j-GFP12 (CasΦ-3) Assay Description: A plasmid expressing CasΦ and gRNA tranfected into stable GFP cell line in triplciate. 72 hours post transfection, GFP negative cells were quantified by flow cytometry Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000059 Editor CasΦ-3 Delivery System Lipofectamine 3000 Delivery System Subtype Commercial Reagent Guide Cas12j-GFP12 (CasΦ-3) Vector pPP444; Model Species Human Model Name HEK293-TgEF1a-eGFP-BSD Application Method lipid transfection, after 24 hours add Puromycin selection Application Site Dosage 200 ng Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format plasmid Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of GFP negative cells Mean 0.96  1 1.2  2 0.79  3 0.8999999999999999