Experiment: Testing newly discovered Biggie Phage editors in human cells
PI: Jennifer A Doudna, PhD Jillian Banfield, PhD
Editing Assay:
A plasmid expressing CasĪ¦ and gRNA tranfected into stable GFP cell line in triplciate. 72 hours post transfection, GFP negative cells were quantified by flow cytometry
Parent Project: Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Download: Submitted files
Publications:
- CRISPR-CasĪ¦ from huge phages is a hypercompact genome editor. NCBI
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
Editing Efficiency |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Condition |
Sex |
Editor |
Model |
Delivery |
Target Locus |
Guide |
Vector |
Dosage |
% of GFP negative cells |
Image |
Associated Publications |
Publication Title |
---|
CRISPR-CasĪ¦ from huge phages is a hypercompact genome editor. NCBI |