Experiment Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Experiment Condition Male Assay Description: Editing efficiency assessed by quantifying tdTomato positive area above threshold Tissue Measured: cerebral cortex Cell Type : astrocyte Measurement Type: Editing Efficiency Record ID 15000000949 Editor SaCas9 Delivery System Delivery System Subtype Guide L1-modified; R2-modified Vector BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2; BI28:AAV-GFAP-SaCas9-WPRE3-pA; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method Intravenous (retro-orbital sinus injection) Application Site central nervous system Dosage 3e11 vg each virus Injection Frequency once Injection Rate Injection Volume 50-100 µL Days post injection 4 weeks Editor Format AAV encoded Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: tdTomato positive area Mean 38.7  1 41.8  2 60.1  3 27.4  4 25.5